Enzyme Hydrolysis using IMCSzyme for Pain Management Urine Drug Testing


A method comparison of hydrolysis parameters which used abalone extracts to a dilute-and-shoot process using IMCSzyme, indicated IMCSzyme had comparable or better recoveries. The glucuronide metabolites in patient samples were recovered better with IMCSzyme despite the decrease in enzyme volume and incubation time. The study demonstrated that IMCSzyme is a viable and cost-effective alternative to their previous hydrolysis procedures using abalone β-glucuronidase.


Figure 1
Figure 1. Codeine, morphine, oxymorphone, fentanyl, tramadol, cyclobenzaprine, amitriptyline, naloxone, showed higher quantities following hydrolysis with IMCSzyme, indicating more complete conversion than was previously obtained with abalone β –Glucuronidase.


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Summarized from Independent technical poster presented by Agnes Cua - Precision Toxicology at MSACL 2015