Evaluation of Enzymatic Hydrolysis Efficiencies for Amitriptyline and Cyclobenzaprine in Urine

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  • Evaluation of Enzymatic Hydrolysis Efficiencies for Amitriptyline and Cyclobenzaprine in Urine
ABSTRACT:

A summary of the JAT article: “Variations in enzymatic hydrolysis efficiencies for amitriptyline and cyclobenzaprine in urine”

A collaborative study with researchers affiliated with Department of Chemistry and Biochemistry at the University of South Carolina was conducted to investigate discrepancies in recoveries of two commonly prescribed compounds, amitriptyline and cyclobenzaprine, in patient urine samples when hydrolyzed with different enzymes from different sources. A 2- to 10-fold increase in analyte recoveries was seen for patient samples hydrolyzed using a genetically modified β-glucuronidase (IMCSzyme®) over samples hydrolyzed with β-glucuronidase from Haliotis rufescens. Outcomes reported from four commercially available β-glucuronidase enzymes (IMCSzyme®, Patella vulgata, Helix pomatia and H. rufescens) on patient samples that tested positive for amitriptyline and cyclobenzaprine.

Results confirm reduced hydrolysis of glucuronides by β-glucuronidases isolated from mollusks, but near complete conversion when using the genetically modified β-glucuronidase (IMCSzyme®).

 

Reference:
Kaylee R. Mastrianni, L. Andrew Lee, William E. Brewer, Nagarju Dongari, Michael Barna and Stephen L. Morgan (2016)Variations in enzymatic hydrolysis efficiencies for amitriptyline and cyclobenzaprine in urine. Journal of Analytical Toxicology doi: 10.1093/jat/bkw062.

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